由于密封套圈尖端是一样的，不同制造商的色谱柱柱头螺纹深度不一致，可能会出现拧紧后色谱柱端面无法与PEEK垫片齐平，但不影响使用。

栓接流程

将在线过滤器直连上色谱柱

1.国际标准色谱柱连接
          手动拧紧在线过滤器后请再使用扳手旋拧30°。
        2.非标准色谱柱连接

           较深螺纹柱头色谱柱可将PEEK垫片取下来手动拧紧后再使用扳手旋拧15°。
           *如发现将PEEK垫片取下后依然无法零死体积连接，则产品与色谱柱柱头螺纹不匹配。

注意：请小心安装，不要过度旋拧在线过滤器，金属密封套圈产生的扭力可能会损坏色谱柱柱头螺纹。

Designed for NanoLC, Not Scaled-Down HPLC

At nanoflow rates, even a small internal volume can have a measurable impact on chromatographic efficiency. This kind of Guard Filter is engineered around the requirements of modern nanoLC systems:

1. Low internal volume as 10 nL to minimize extra-column dispersion
2. Precision filtration to prevent particulate contamination
3. Optimized flow path for stable nanoflow operation
4. High-pressure construction up to 1,200 bar for nano UHPLC applications
5. Bioinert porosity filter for improved sample recovery
6. Zero dead-volume to build in Valve port and Column

Built for LC-MS and Proteomics

The filter is particularly suitable for applications where chromatographic efficiency and peak capacity are critical, such as Single-cell proteomics and Spatial proteomics. When routinely run these samples, filter is much better for sample recovery than a trap column. If you are an intact protein researcher, detergents are widely used during protein extraction, solubilization, membrane-protein preparation, and other sample processing steps. Detergent-containing contaminants interception is inevitable for downstream nano LC MS analysis.

滤片更换

• 旋开在线过滤器底座，更换滤芯，滤芯片安装无方向要求。

• 更换结束后手动拧紧底座，再使用扳手旋拧180°使得底座圆环轻触滤片塑料环实现密封。