In order to prepare high quality peptide samples for LC-MS analysis, it is very important to ensure excellent overall quality of proteomic experiments. Peptide samples collected after enzymatic digestion require treatment to remove salts, possible pieces of gels or particles that can damage the equipment or falsify the results.
Preciex® Desalting Stage-tip is the ideal tool for this application as well as for drug extraction or small molecule analysis in urine or serum in bioanalysis.
Several capacities are available to help you in your purification protocols.
Our application notes have shown that our Desalting tip columns are the perfect tools for peptides desalting with excellent results.
Stagetips, centrifugeable, compatible with automates for high throughput screening, useful for biomolecular applications of peptide desalting, proteomics, and biomarker discovery.
Process of use: centrifugation, vacuum, positive pressure.
Formats: 10µL, 200µL, 350µL and 1mL.
For any other format or online columns, contact us.
Features of Desalting Stage-tip:
• High recovery for concentrating and purifying peptides, proteins and oligonucleotides
• High binding capacity with fast-flow tip format
• Extensive formats for processing fmol to pmol of sample
• Several chromatographic beds for treating different sample
Common product Specifications:
Sample | peptide | oligonucleotides | protein |
Chromatographic bed | C18, 100Å | RP18, 200Å | C4, 1000Å |
Volume | 200μL | 200μL | 200μL |
Capacity | 2ug | 2ug | 2ug |
pH Range | 2-12 | 2-12 | 1-10 |
Temperature | 4-70°C | 4-70°C | 4-70°C |
Quantity | 96 Tips | 96 Tips | 96 Tips |
Detection Method | HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy |
Ordering Information:
Description | Format | Capacity | Part Number |
Preciex Desalting C18, 100Å | 10μL | 2μg | 2106021 |
Preciex Desalting C18, 100Å | 200μL | 10μg | 2106102 |
Preciex Desalting C18, 100Å | 200μL | 30μg | 2106303 |
Preciex Desalting C18, 100Å | 350μL | 100μg | 2106104 |
Preciex Desalting C18, 100Å | 1000μL | 500μg | 2106505 |
Preciex Desalting RP18, 200Å | 10μL | 2μg | 2102021 |
Preciex Desalting RP18, 200Å | 200μL | 10μg | 2102102 |
Preciex Desalting RP18, 200Å | 200μL | 30μg | 2102303 |
Preciex Desalting RP18, 200Å | 350μL | 100μg | 2102104 |
Preciex Desalting RP18, 200Å | 1000μL | 500μg | 2102505 |
Preciex Desalting C4, 1000Å | 10μL | 2μg | 2101021 |
Preciex Desalting C4, 1000Å | 200μL | 10μg | 2101102 |
Preciex Desalting C4, 1000Å | 200μL | 30μg | 2101303 |
Preciex Desalting C4, 1000Å | 350μL | 100μg | 2101104 |
Preciex Desalting C4, 1000Å | 1000μL | 500μg | 2101505 |
Specialty Desalting Tip-column below are for applications on Evosep one system, which capacity is less than 1ug.
Description | Quantity | Unit | Part Number |
CapaSil Tip-C18 | 96 tips | 10/Pk | 2012710 |
CapaSil Tip-C18 | 96 tips | 50/Pk | 2012750 |
CapaSil Tip-PRP | 96 tips | 10/Pk | 2012810 |
CapaSil Tip-PRP | 96 tips | 50/Pk | 2012850 |
User Guide
https://www.freshbioscience.com/public/upload/202301/20230102161406.pdf
Material Safety Data Sheets (MSDS)